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As imaged in a transmission electron microscope, the powder consists of nanostructured hollow carbon shells, termed carbon nanoshells, that are positive replicas of the nickel particulates.
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This phantom consists of a positive replica of human skin; fabricated using a negative imprint of skin obtained by impressing wax on the skin surface [ 76].
For more precise quantification, a micro-impression of an initial scratch using either resin composite [ 20], where the negative is observed under a scanning electron microscope (SEM), or simply a positive replica from a rubber impression, then observed under a SEM, will both produce very accurate results.
To test whether the two proteins interact, positive colonies were replica plated on SD (leu- ura-) plates containing dextrose and galactose, respectively, and incubated at 37°C for 6 10 days.
Positive transformants were replica plated on SC media lacking maltose but containing 60 mM glucose, fructose, galactose or mannose and recovery of EBY.W4000 growth was assessed after 3 days at 30°C.
Only RT-PCRs, which were positive in all three replicas, negative in the RT- and confirmed by sequencing analysis were considered as positive.
To further minimize experimental error and limit false positive results, three biological replica experiments were prepared and independently analyzed.
PDMS replicas with either positive or negative features from the master mould were attainable.
The fabrication of working stamps comprises two successive replication processes (basically two adapted UV-NIL processes): (1) negative replication of silicon master, and (2) replication of the negative replica to obtain a positive working stamp.
We can then use this negative replica to produce a positive working stamp following the same procedure (Fig. 1 a c).
Each block contains 23 types of positive controls (most of them spotted in 6 replicas) and 3 types of negative controls.
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