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Unexpectedly for a 3D pooling scheme, for the position C170T EMS mutation in SleIF4E exon 1, four positive pools were observed (X12, Y7, Y8 and Z5).
For positive pools, individual sample extracts were then tested to confirm positivity.
Individual blood samples corresponding to the positive pools are then tested by BVDV-antigen ELISA.
It involves estimating the relationship between the probability of a positive pooled test result (dependent variable) and the expected number of infected individuals in a pool (explanatory variable).
Next, we sib selected the positive pools and screened individual lines several times.
Biomolecular analyses were performed on aliquots of 1,789 mosquito pools (Table 2) giving the subsequent results: 19 WNV-positive pools (sampled in 15 stations), 48 USUV-positive pools (sampled in 24 stations) and 8 positive pools for both viruses (sampled in 7 stations) (Figure 1, Figure 2).
The simultaneous circulation of USUV and WNV in Emilia-Romagna was demonstrated by the presence of positive mosquito pools in the same area and in the same period and also by the presence of mosquito positive pools for both viruses.
Sixty-one (18%) of the positive pools confirmed with at least 2 out of 4 duplexes scoring per pool (Figure 5A, see Table S1 for the gene list).
Positive pools were individually retested.
The one laboratory that uses pooling retests positive pools individually.
However, some probes gave more positive pools per pool type.
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