Sentence examples for positive nested from inspiring English sources

In the current study, all positive nested PCR findings were sequenced in order to eliminate the query of false positives due to nested PCR.

Cases of trichomonosis were diagnosed on the basis of the presence of necrotic ingluvitis lesions in combination with positive culture of motile trichomonads and/or positive nested PCR amplification.

The positive nested PCR products were purified, cloned and sequenced.

The positive nested PCR products were subjected to direct automated DNA sequencing without further purification.

Positive nested PCR products were identified by sequencing with the AF2F AF2R primer pair.

Eschar scab in scrub typhus yielded positive nested PCR result for O. tsutsugamushi in a child 7 days after commencement of treatment with azithromycin (7 ).

However, some donors had a weak S/CO ratio (between 1 and 2) with positive nested-PCR tests.

Positive nested-PCR products were purified with Sephacryl 400 resin (Ilustra-MicroSpin S400 HR Columns) and sequenced in a single direction.

Preemptive therapy was used to prevent CMV disease and was initiated upon a positive pp65 cell/3 × 105 PML result ≥1 and/or two or more consecutive positive nested-PCR results.

Both types of cases were further confirmed to be caused by M. tuberculosis complex organisms by positive nested-PCR based on the amplification of IS6110 as described earlier [ 22].

On the other hand, in the positive nested-PCR animals that did not reveal NPME (84.6%—11/13), it can be assumed that the presence of proviral DNA in some samples may have been due to amplification of the genetic material from the intraluminal lymphocytes of cerebral blood vessels, taking into account that around 30%70%% of infected animals have shown persistent lymphocytosis [ 23].