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Kim Clijsters whacked her racket against her thigh as if trying to prod her legs back to life, and she mumbled positive lines to herself that seemed tugged out of a fortune cookie.
The positive lines (RNAi1 and RNAi2) with down-regulated GNS4 expression displayed a semi-dwarf stature (Fig. 3a, c).
Twelve transgenic plants were generated, and all the positive lines showed complementation of the mutant phenotypes (Fig. 2d f).
DNA analysis using Pi2/9-RH identified 24 positive lines in 55 shortlisted landraces which showed resistance to 4 rice blast isolates.
Then, sequences of Pi2/9 homologues in Pi2/9-RH positive lines helped the clustering of identical haplotypes to avoid redundant functional characterization.
Twenty-two positive lines were identified and semi-quantitative RT-PCR was performed to determine the expression levels of BnaABF2 in T3 homozygous transgenic plants.
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We checked three independent hygromycin-positive lines (#1, #3, and #4).
Low cytotoxicities were observed in all cell lines, while transgene activity promoted by folate-tagged lipoplexes in FR-positive lines was tenfold greater than that by untargeted constructs and cell entry by folate complexes was demonstrably by FR mediation.
We observed that suppression of CSNK1E led to reduced proliferation in three of the β-catenin-positive lines, HS578T, BT474, and BT549 but did not significantly affect the β-catenin-negative line, SKBR3 (Fig. 3D, E).
In addition to the downregulation of pro-IL-1β, HPVs specifically downregulated the genes encoding inflammasome components NLRP2 in three of the four HPV-positive lines (Fig. 6C) and PYCARD/ASC, but not NALP3, possibly contributing to the observed lower level of IL-1β.
As the initial RNAi screens were limited to three β-catenin-positive lines and one β-catenin-negative line, we tested the hypothesis that CSNK1E is preferentially required in additional β-catenin-positive cells by determining the effects of its suppression in an expanded panel of breast cancer cell lines.
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