Exact(5)
A positive LAMP result was obtained for 9 of the 24 confirmed cases and for 12 of 94 suspected cases.
After addition of SYBR green I dye the positive LAMP reactions turned green while the negative ones remained orange.
Positive LAMP results were identified by the addition of 120 μM hydroxynaphthol blue to each test reaction pre-incubation.
In comparison to the PCR assay, all of the 47 strains generated positive LAMP results (Table 1).
A positive LAMP result was obtained for 8 of the 10 confirmed cases (sensitivity, 73%; specificity, 100%), while none of these samples were positive by nested PCR (sensitivity, 0%; specificity, 100%).
Similar(55)
However, when LAMP-2 was knocked down, approximately 40% of bacteria were no longer positive for LAMP-1 (Fig. 3D, panels vii inset, viii and Fig. 3E).
In addition, positive RT-LAMP results could be confirmed through the production of white magnesium pyrophosphate precipitate or through mixing the fluorescent dye GeneFinder™ with the amplification products.
Specifically, 28±8% of T. whipplei phagosomes were positive for Lamp-1 at day 0; this percentage increased to 71±9% and 94±6% after 3 and 12 days, respectively.
Coinciding with their conversion into amastigotes, the number of promastigote PVs positive for LAMP-1 increased from 20% at 1 hour, to 46% by 24 hours, (P<0.001, Chi square).
Similarly, the false positive RT-LAMP result could be a true positive but need to be verified by virus isolation.
After 30 min internalisation, some of the ovalbumin-positive punctate structures stained positive for LAMP-1 (Fig. 5B), indicating the delivery of ovalbumin to the late endosome/lysosomes.
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