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There was one false positive insertion called at u =1.
The first constraint was applied to eliminate false positive insertion predictions due to a breakdown of the RRL resulting in shorter spans of paired-end reads, whereas the second constraint was applied to reduce the number of false predictions due to sequencing errors.
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By using a 5,000 bp alignment window, on average ∼2,116 positive deletions and ∼69 positive insertions were reported per strain, the largest being 4,916 bp long.
However, the deletion variant was not associated with odds of OvLV infection (all P > 0.05 for estimable animal sets with more than 35 OvLV-positive insertion homozygotes; see Table S3).
Thus without careful processing of DNA sequence data, the direct mapping of insertion site sequence reads to the genome may result in the identification of false-positive insertion sites.
Worthy of note is the low template-dependent fidelity score for Kapa HiFi (Table 2), where we detected a number of false-positive insertion calls mainly in regions of TATA repeats.
Although s65956 was not associated with proviral concentration in animal set 2 (P > 0.52) in a previous GWAS (White et al. 2012), the ZNF389 deletion variant was associated with proviral concentration in all the animal sets with more than 35 OvLV-positive insertion homozygotes (Table 2; Fig. S1).
This phenomenon may result in unlikely positive reactivity insertion accident, affecting the safety performances of the reactor itself.
In the current study two positive reactivity insertion transients are considered, SCRAM protected and self-limiting power excursion cases.
Voiding the core from its LBE coolant resulted in a positive reactivity insertion which is typical of most fast reactors.
We present two different ways to implement this technique, together with an overview of standard methods, and we apply them to a positive reactivity insertion transient.
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