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In order to synchronise the FISH status of cytological and histological specimens, we tuned the threshold of a positive FISH result for cytological specimens using ROC curve based on the 33 cases with matched specimens (Linden, 2006).
A positive FISH signal was defined as fluorescence in excess of that detected with the negative control FISH probes.
Six fish species were found to have VHSV and most positive fish were round goby and yellow perch.
Tissue samples from qRT-PCR positive fish were shipped to the Western Fisheries Research Center (Seattle Washington) on dry ice.
The qRT-PCR titers in the 55 positive fish were highly varied (1 to 5,950,000 N gene copies).
Four of the 55 qRT-PCR positive fish were confirmed VHSV positive by cell culture analyses (Table 1).
Similar(26)
All tumours selected as IBC were axillary node-positive, oestrogen- and progesterone-receptor-negative, as evaluated by immunohistochemistry (with positivity cut-off value of 1%), P53-positive and HER-2-positive FISH amplification/Ventana Benchmark ⩾6) grade 3 ductal carcinomas with inflammatory stroma and prominent vascular invasion.
Largemouth bass virus was not detected in biofilms or water from ponds that had previously contained LMBV-positive fish, suggesting either that the viral concentrations were below the detection limit of qPCR or that the fish represented the main LMBV reservoir.
In other Tettigoniidae, single rDNA-positive FISH signals have been observed in European representatives of Saginae and Bradyporinae [ 32, 35].
In contrast, when pT2/ S2EF1α-GM2 was used, most of the GFP-positive fish exhibited fairly strong, ubiquitous expression.
The paraffin-embedded TPC-1 cell line showed rearrangement-positive FISH signals in 97% of nuclei counted (100 out of 103 cells scored).
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