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Lane 3: positive control from pHT01-kIspS.
A positive control from brain tissue revealed a band at around 250 kDa.
The willow extract was incubated with normal mononuclear cells as a positive control from healthy volunteers (6 samples).
A positive control from a young rat articular cartilage joint was also included with each run.
Ten percent of the input was saved as a positive control from the total lysate.
The previous analysis used the MIA-Pa-Ca-2 cell line as a positive control from which to compare relative expression of DCD mRNA.
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We attempted to PCR-amplify each locus on the extracted CAS lesions and three positive controls (from a Darwin's finch, a yellow warbler, and a Galapagos mockingbird) from [12].
The light microscopy and fluorescence microscopy were described elsewhere [22], [58], in which we performed relevant controls such as absence of the primary antibody, negative controls from uninfected animals, positive controls from kidneys of WNV-infected animals and WNV-infected Vero cells, and confirmation of antibody specificity using different staining protocols.
Positive controls from the collection of the Biotechnology Research Centre, Islamic Azad University, Iran, were included in each PCR reaction, while sterile distilled water was used as the negative controls.
Serial positive controls from seven standard antigens with known concentrations were added in duplicate in microplate wells, and a negative control also added in duplicate.
PCR was performed using primers and positive controls from J.S. Opp and V. Young (University of Michigan) following the method of Eaton et al. (2011).
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