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CoMFA and CoMSIA models suggested that a positive bulk with hydrophobic effect is desirable around position 4 and 5 and hydrogen bond acceptor groups around pyrazolones ring will be helpful.

Whereas, being shrunk of the cavity due to an external compressive force indicates the positive bulk modulus in a conventional case.

Therefore, the threshold prevalence (50%%) estimated in the dilution experiments to have positive bulk milk appears correct.

Using this system, new infections are detected and the proportion of false positive bulk milk samples could be defined for the SVANOVIR ELISA.

For example a positive bulk tank milk sample indicates that herd-level vaccination is not necessary as natural immunity is present.

Further studies based on a metagenomic approach will be able to confirm or exclude the presence of Helicobacteraceae in these positive bulk tank milk samples, providing also an identification at species level.

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Similar(52)

Two-by-two table analyses revealed that the presence of mycoplasmas in bulk-tank milk increased the risk of surpassing all SCC thresholds considered, with the highest risk for Mycoplasma positive bulk-tank milk samples exceeding the threshold of 1500 × 103 cells/ml (odds ratio = 2.42 (1.49 < OR < 3.91).

Stable transfectants were selected by culturing in 400 µg/ml G418 and sorted for GFP or Podocalyxin-positive bulk populations or clones using a BD FACS Vantage.

(C and D ) Analysis of the membrane area of HRP reaction product-positive bulk endosomes per cross-sectional area of nerve terminal in each of the conditions investigated.

The large fraction of HRP reaction product-positive SVs observed in all genotypes during the recovery period documents formation of these vesicles from HRP reaction product-positive bulk endosomes formed during stimulation.

CD70-positive bulk cultures were cloned by limiting dilution cloning and grown for at least 4 weeks in growth media without selective antibiotic to identify CD70-positive stable clones.

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