Immunostaining for F4/80 positive macrophages revealed no significant difference in the F4/80 positive areas between UUO (or sham) kidneys from Sigirr-deficient and wildtype mice (8.0±0.7% versus 7.6±0.7%, Figure 5).
We quantified intrarenal myofibroblasts by immunostaining for smooth muscle actin (SMA) but could again not detect any difference in the SMA positive areas between UUO (or sham) kidneys from Sigirr-deficient and wildtype mice (21.7±2.1% versus 22.0±1.8%, Figure 5).
At week 8 and 12, there were significant increases in neurofilament-positive area between the groups of NAT-NGF and LBD-NGF compared with the control (n = 6, P<0.05) (Figure 5N).
There was no difference in the βIII-tubulin-positive areas between culture conditions I and II, right panel).
IL-32 and IL-17 were co-localized near the TRAP-positive areas between the cartilage and tarsal bone of both mouse models.
In conclusion, there is significant association between amyloid positive area in renal tissue and renal function, especially Cr and eGFR.
In contrast, we found positive area-sensitivity on between-years site colonisation for this latter species at small spatial scales.
The analysis of these paired sections demonstrated a linear correlation between the positive areas of both proteins in each specimen (Fig. 5F, bottom left).
We also found a correlation between the CD34 positive area fraction and C4d positive endothelial area fraction in diffusely infiltrating astrocytoma samples.
Although no significant differences in omental CD31 positive area were observed after seven weeks between groups I and III, a declining trend in the CD31 positive area upon paricalcitol treatment was observed (33% versus 14%and32%2% versus 27% for groups I versus II and III versus IV, resp.; data not shown).
PTEN positive areas were seen between muscle fibres in both PTEN+/+ and PTEN−/− muscles, suggesting that PTEN was largely restricted to cells of endothelial origin.
