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A total of 298 positions sequenced for one individual from each of the 19 Cubitermes colonies, for Apilitermes longiceps and Crenetermes albotarsalis were aligned for ITS2 sequences.
Mean MR was first calculated considering all positions sequenced in this study (853 bp) and then considering all heteroplasmic sites (Table 1).
In Brassica, we reanalysed the data in [ 26] but restricted the dataset to the codon positions sequenced in A. halleri and excluded two A. lyrata sequences used in this study.
At heterozygous positions sequenced bidirectionally, the minimum number of original template molecules required to produce a signal is only four: forward reference, forward alternate, reverse reference, and reverse alternate.
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Finally, because some identifications are based on single nucleotide positions, sequencing errors could cause further false identifications.
Information on physical positions, sequences accession numbers and molecular characteristics were retrieved from corresponding web resources [ 58, 66].
PROCAIN combines residue substitution constraints at individual sequence positions with sequence motif matching, residue conservation and SS scoring.
In these SNP/INDELs position, sequence of KYJ is same to Nipponbare.
According to formula (7), the corresponding saturation position sequence [d 1, d 2, …, d n ] has been calculated.
We additionally measured the position sequence of the handles using the motion capture system.
Each job of the consensus permutation is inserted into a new sequence according to the position sequence.
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