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American geneticist Charles Yanofsky showed that the positions of mutant sites within a gene matched perfectly the positions of altered amino acids in the amino acid sequence of the corresponding protein.
The RNA sequences were named based on the original sequence name and joined with the positions of mutant nucleotides in the original sequences.
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DOI: http://dx.doi.org/10.7554/eLife.01834.018 10.7554/eLiFigure34.019 Figure 6 figure supplement 1. Positions of MtMscL mutants at the proximity of the narrowest pore constriction.
Predictably, the severity of inter-strand bundling disruption depends on the mutant:wild-type ratio protein concentration and on the relative position of the mutant protein incorporated into the actin filament (Fig. 6e).
In Figure 5B and 5B', the apical and basal sections of a wing pouch are shown in which an lgl mutant clone is being basally extruded (in Figure 5B the position of the mutant clone is outlined), which also showed increased pJNK staining.
The position of this mutant in the middle of the second beta strand of the small beta sheet of the N-terminal domain is shown in Fig.1C.
The deduced active site structure (TSTTHKTL) and the position of the mutant (249 isoleucine) were briefly presented the three-dimensional structure of AnSHMT.
While BSA (step 3) provides a rough chromosomal location, it is still necessary to substantially refine the map position of the mutant of interest for the eventual cloning of the gene.
The JAK2 V617F detection system comprises a single set of primers and two probes that differed only at the position of V617F mutant nucleotide substitution and fluorescent labelling, so as to discriminate the mutation from the wild-type sequence in a single qPCR reaction.
For both positions of the selected mutant, we simulated with selection coefficients of 0.1, 0.05, 0.01 and 0.005.
Recent studies suggest that a non-physiological intermolecular disulfide bond between cysteines at positions 6 and 111 of mutant SOD1 is important for high molecular weight aggregate formation in cells [27].
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