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The positions of adhesion sites were marked by paxillin staining.
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It is possible that IFT exerts force on cell-cell adhesion sites and determines the positioning of these adhesion sites by moving them along the length of the cilium.
The cells stained with fluorescent dye of DAPI were used to observe the position of cell adhesion on the modified line-width.
The determined initial contact position DC, equilibrium separation Deq, position of maximum adhesion DAdh, and thickness in the absence of ligand DT are averages of 7 20 measurements from 2 3 independent experiments.
This study was designed to investigate the regulation of cell-adhesion strength by the size and position of focal adhesions (FA).
Paxillin is a focal adhesion-associated adaptor protein and it is used to indicate the position of focal adhesions.
We show how geometry guides the spatial positioning of focal adhesions to maximize interaction with the matrix, and uncover a relationship between αvβ3, α5β1 and mechanochemical regulation of osteogenesis.
Simultaneous staining for vinculin and STIM1 revealed the relative positioning of focal adhesions and ER PM junctions.
In specific experiments aimed to reveal dynamics of focal adhesions (e.g. Fig. 3A and corresponding text in the Results section), all images were equally thresholded and subjected to single pixel size "remove outliers" function using ImageJ software to eliminate cytosolic component of fluorescence and highlight the position of focal adhesions.
To complete these processes, they modulate their physical properties including rigidity, position, motility, and strength of adhesion (Fagotto, 2014).
The effect of body thickness on the curve of the maximum adhesion and the position of the minimum adhesion point is shown in Fig. 15.
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