Poorly positioned sites presumably introduce clashes or strain into either the complex or DNA which will, in turn, reduce the stability of the complex.
In addition, we show that closely positioned sites tend to be activated by the same kinase.
Linkage is only detectable between closely positioned sites (or it may be weakly inferred between a pair of sites if intermediate sites are linked).
The positioning mechanism involves Min proteins, which are homologous to those Min proteins used to position sites of division in modern-day bacteria (Margolin, 2005).
Further, we analysed the divergence sites by positioning these sites on the 3D structures of GGT proteins of E. coli (2DBX) and human (4GDX).
A factorial ANOVA compared the main effects of fruit position, site and cultivar type, and the interaction effect between fruit position, site and cultivar type on fructose, and glucose contents.
However, avoiding CDRs counteracts the requirements proximity to the linker-thiol and hapten-positioning site.
Therefore, we named this pocket as the second substrate-binding site (Site II) with respect to the placeholder position (Site I).
Of these highly conserved Plants positions, site 13 was also highly conserved in Fungi, while sites 10 and 12 were only moderately conserved in Animals and Fungi.
Evidently, some advantages exist for positioning PTM sites within disordered regions.
Rather, it is more likely that the lariat production contributes to backsplicing catalysis by positioning splice sites.
