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We conclude that the divergence of the Chrysogenum complex occurred ≈0.75 MYA with more recent divergences arising ≈0.5 MYA; however the precise relative positioning of species could not be inferred with strong statistical support.
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Moreover, the position of species along the positive side of axis 2 followed a body size increase among endogeic species.
The geographic extent and position of species ranges were reconstructed using geographic information systems (GIS; Fig. 1).
Although the exact position of species within the Callicebus genus is debated, the white-eared titi has been placed within the subgenus Callicebus in the C. donacophilus group with the Rio Beni titi (C. modestus), Rio Mayo titi (C. oenanthe), Ollala Brothers' titi (C. olallae), and white-coated titi (C. pallescens).
We also determined full plastid-encoded rbcL gene sequences to infer the phylogenetic position of species.
The relative position of species within the Dianthus clade was the least resolved, suggesting recent radiation of Dianthus species.
The main aim was to explore the phylogenetic positions of species of the Amitostigma alliance within the tribe.
Our results show that, in general, the geographic position of species' ranges display a stronger phylogenetic signal than their size.
Compared to previous reports, we found some differences in geographic positions of species borders and contact zones.
This proportion is independent of the phylogenetic position of species and is not higher in the post-WGD (whole genome duplication) species S. cerevisiae and C. glabrata.
The systematic position of species not included in the phylogenetic analysis was assessed based on the relationships suggested by Remsen [ 7] and Marantz et al. [ 41].
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