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The injections did not alter positioning of nuclei.
The unusual positioning of nuclei often, but not always, corresponded with multinucleated cells.
Cooper et al (2004) reported peripheral positioning of nuclei in mouse myofibers, although triad organization was not studied.
Therefore, our results suggest a novel role for the positioning of nuclei in the regulation of asymmetric division.
Examining the positioning of nuclei along myofibers, we found that in P1-deficient cells nuclei were no longer distributed in a regular pattern all along the fibers, but often showed clustering.
Taken together, these data demonstrate that transgenic expression of either DVAP-V260I or DVAP-WT in neurons as well as in muscles elicits the formation of aggregates and a severe disruption in the architecture, size and positioning of nuclei.
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The roles of the peripheral positioning of nucleus have not been analyzed previously in C. elegans.
In direct molecular dynamics, the positions of nuclei are changing with time and the electronic structure adjusts following the time-dependent Schroedinger equation (TDSE).
The positions of nuclei were shown by DAPI staining.
Our analyses of the positions of nuclei demonstrate that the rate of expansion is consistent between muscle cells along the entire length of the tail during tail extension.
In various polarized cells, positions of nuclei are often off-center.
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