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However, among remodelers striking differences have been observed concerning their preference of nucleosome repositioning on short stretches of DNA containing nucleosome positioning elements.
The question concerning the sequence properties of these nucleosome positioning elements is perhaps even more interesting than their distribution along the genome.
In particular, as they appear to generally have all their cis-elements located upstream of the cleavage site and instead of A(A/U UAAA hexamers, often have A-rich positioning elements (Supplementary Figure 5).
This is likely to be a conservative test for purifying selection because many synonymous sites are under selective constraint in S. cerevisiae (Zhou et al. 2010), and intergenic regions are likely to contain constrained sequences other than TFBSs (e.g., nucleosome positioning elements, noncoding RNAs etc).
One of my favorite pieces of complexity comes from a U.K. consulting firm that claims a brand has nine positioning elements in a customer's mind: functional needs, objective effects, functional roles, attributes, core evaluators, psychological drives, psychological roles, subjective character and psychological needs.
These positioning elements frequently occur in the vicinity of promoters and enhancers, and a variety of direct functional studies provide strong emerging evidence that they directly regulate transcriptional initiation and other genomic functions as well.
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NCPs containing the various FRET pairs were reconstituted with the 601 DNA positioning element.
Regarding this trend, what would be the best positioning element for the global brands having Western-origins in the emerging Asian markets?
I guess poor customer service is a core MacMall positioning element that I could have known about ahead of time.
The 1 kbp bare DNA fragment without nucleosomal positioning element was obtained from Eurogentec.
We revealed that the newly adopted nucleosome positions after enzymatic remodeling are influenced by the underlying DNA sequence by inserting DNA fragments into the 601 positioning element.
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