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Consider now that the polymerase active site is positioned at the fork of the flap (Figure 4B).
Based on this hypothesis, the rigid PolI model is schematically shown in Figure 2. Consider that the polymerase domain has just synthesized a transient flap, with the DNA substrate binding to the polymerase domain, where the polymerase active site is positioned at the fork of the flap (see DNA substrate with solid lines in Figure 2).
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We begin with the polymerase active site positioned at the replication fork, with the downstream 5'-flap being very far away from the 5'-nuclease domain (Figure 4A).
"Somewhere at the fork of a highway, I ended up on the wrong fork," he recalled.
Its location at the fork of the Red River and the Red Lake River gives the city its name.
The lanes rejoin as they approach the Don Mills Road interchange at the "forks of the Don".
Through the 1820s a small settlement grew around Wolf Point, at the forks of the Chicago River.
Governor Dinwiddie then sent Captain William Trent to build a fort at the Forks of the Ohio.
London is at the forks of the non-navigable Thames River, approximately halfway between Toronto, Ontario and Detroit, Michigan.
The North and South branches of the Thames River meet at the centre of the city, a location known as "The Forks" or "The Fork of the Thames".
Find the fork of the saddle.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com