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Subcutis/muscle electrodes were two monopolar needles, 0.3 mm in diameter (teflon isolation except for 2 mm at the tip), inserted vertically, 1.5 cm apart, just below the skin surface for subcutis measurement and deep under the fascia for muscle measurement (intramuscular position verified by observing electrode movement under voluntary contraction and/or low-intensity electrical stimulation).
For those employed at academic institutions, the institutional site was checked and the employment position verified as of February, 2010.
This allowed the placement of the electrodes in the mid-pharyngeal area, a position verified by a VFS-still images following insertion (see Supplementary material).
At the conclusion of the surgery, all patients will have insertion of a 'Pain buster' catheter into the subacromial space with the position verified by direct or arthroscopic vision.
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Treatment will be setup using reproducible positioning, verified using an on-line protocol, for all patients in this study.
An oesophageal Doppler probe (CardioQ™; Deltex Medical, UK) was inserted, connected to the CardioQ-ODM™ monitoring system and correct positioning verified.
Two main metabolic pathways of Pra-D and -Pra-E were shown to be oxidation and hydrolysis, and substituent at the C-3′ and C-4′ positions verifies the level and the type of reactions.
Intracerebroventricular cannula position was verified by a positive dipsogenic response to ANG II (150 ng/rat).
The calculated depth of the library at each position was >1000-fold >1000-foldresence of andegeneratheNNS codon at each presence was verified by sequencing.
Their position was verified by a United States Air Force plane.
Purification of the products by several chromatographic steps was reported, and the 13C labeling position was verified using 13C NMR.
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