Exact(3)
Reads from the mate pair libraries were reverse complemented prior to alignment using fastx_reverse_complement (http://hannonlab.cshl.edu/fastx_toolkit/). Duplicate sequences were removed and reads were position sorted using Picard version 1.48 (http://broadinstitute.github.io/picard/) with the default parameters.
First and foremost it's crucial that we get the manager's position sorted out so they've got the best chance possible to have a really good start to life in League Two next year".
The sorted BAM files were further processed in the variant calling step: using the "mpileup" function in SAMTools, indexed reference sequences and position sorted bam alignment files generated files with read information at sites where mismatches and indels from the reference sequence were detected.
Similar(57)
During Phase 1 of the study, the seventeen respondents from district and community-level positions sorted booklet cards into three piles according to their perceived importance.
Listed in Table 1 and 2 are the top positions sorted according to their U-values for RSS (stringent cut-off of 15) and Z-scores for CSS (stringent cut-off of 2), respectively.
A position-sorted BAM file can be indexed.
This saves one compression write read step in the common case in which a duplicate marked position-sorted file is needed later in the pipeline, and two such cycles if a duplicate marked read-id grouped file is also needed.
Extant duplicate marking programs such as PICARD MarkDuplicates (http://picard.sourceforge.net/) and SAMBAMBA markdup (https://github.com/lomereiter/sambamba) require position-sorted SAM or BAM (Li et al., 2009) as input, and perform two passes over the input data, thereby requiring their input file be stored on disk.
Table 2 Original positions of sorted target features for a random set and actual transmission.
Table 1 Original positions of sorted source and target features for a random set.
The aligned reads were sorted by genomic position and the sorted BAM file was used as input to REDHORSE.
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