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Since the images are precisely aligned in software, the position of the stage is not critical, and the image acquisition is rapid.
A STEM imaging session started with the adjustment of the vertical position of the stage by focusing on debris on top of the liquid cell using the secondary electron detector positioned above the specimen.
FIB and SEM pictures were aligned using the FIB-SEM intersection (setting the eucentric position of the stage), and the magnification was set between 2500× and 3000×.
To determine the position of the stage green-revertible albino gene sgra in " whs18" on genetic map, an F2 population derived from barley cultivar Edamai No.9706 and whs18 was used.
Without x- z imaging it is generally necessary to step the axial position of the stage while taking scans in order to find the planes where the features of interest are most prominent.
To this end we implemented an automated autofocus procedure that adjusts the position of the stage to compensate the variation of interfacial position caused by water evaporation and drift of the stage during the experiment.
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D24 devices containing BSA were positioned on the stage of a Renishaw inVia micro-Raman microscope for analysis.
The wing grabber with attached fly is then positioned on the stage of a macroscope (see below) and a video image recorded.
Standing-wise, it's all much of a muchness – though the positioning of the stage can sometimes obscure views from the flanks.
For the commonly used precision stage that has six supporting points, there will be different deformation types of the stage when the workpiece is located at different positions of the stage.
On the z-axis stage a picomotor actuator (Tiny picomotor actuator, Model 8353 Intelligent Picomotor driver; New Focus, Micro-Controle Spectra-Physics S.A., Évry Cedex, France) for automated positioning of the stage is implemented, hence facilitating automated positioning of the focal point of the achromatic lens on the cantilever surface.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com