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An identifiable 'tag' is inserted into the host genome, which then allows identification of the position of insertion in the genome via PCR-based techniques.
Briefly, the four detected morphotypes were found by examining all TEM micrographs obtained and inspecting for unique morphologies (i.e., size, shape, presence or absence of flagella, number of flagella, position of insertion of the flagella, presence/absence of electron-dense bodies and its location).
Further studies are needed to determine the optimal insertion distance for a paravertebral catheter, and the optimal position of insertion needle into the thoracic paravertebral space.
Within each element, the location of each pin is determined by the segmental level of the long bone (I proximal to VIII distal), the circumferential position of insertion (clock face, with 12 anterior, 3 medially, 6 posterior, 9 laterally) and the angle of insertion relative to the long axis (e.g. 90° perpendicular).
Hence we performed the preliminary study to determine the position of insertion of the sensor.
The level of activities and the amount of soluble hybrid enzymes produced depend however on the position of insertion.
Similar(45)
The position of insertions can be determined by amplifying DNA flanking the provirus using linker-mediated PCR and mapping the resulting sequences onto the genome.
While the position of insertions to the Bet v 1-like core is identical for both families (between β-strands 1 and 2, between strands 3 and 4, and at the C-terminus), the lengths and secondary structure contents of the insertions differ.
Second, we used site-directed transgenesis to create flies carrying each of the constructs (WT, F57I, I59T, and D67H) at the same genomic location, thus eliminating any effects of different positions of insertion.
Transposable elements (TEs) can move within their host genomes by changing their positions of insertion in a process called transposition (McClintock 1953; Rubin and Spradling 1982; Craig et al. 2002).
For each insertion sequence, the genomic position of the insertion sequence and of the inserted position in the cognate genome are given.
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