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Recombinant Drosha can promote RNA cleavage in vitro, however, the position of cleavage is imprecise [ 41].
EXO1 has been suggested to back up FEN1 during replication [ 18, 19]; therefore we sought to determine the exact position of cleavage of a flap substrate.
Because of high level of conservation of the 5´ head sequences (Table 1), we hypothesize that they might serve as determinants of position of cleavage site.
As a consequence, the position of cleavage into peptides after exposure to the protease pepsin was different for the DHFR variants, leading to nonequivalent peptides for comparison.
Therefore, the uncompromising site-specificity of rhomboid proteases is not an inherent property of the enzyme itself, but rather results from the membrane somehow directing the position of cleavage.
It would also be interesting to investigate how the position of cleavage sites relative to regions of different flexibility affects the steady state level of knotting, as well as looking into how the effect of flexibility may combine with previously suggested topoisomerase II guidance mechanisms.
Similar(54)
The relative positions of cleavage sites would determine exactly which domains colocalize with the LRT.
An intriguing question arises as to whether the correlation between the positions of cleavage sites and DNA flexibility could be important in the regulation of knotting.
So, the experimental data on double digestion of γ-satellite DNA with GlaI and restriction endonucleases are coincided with calculated positions of cleavage sites in a physical map.
The positions of cleavage sites for Fundulus HCE (FHCE1/2) and LCE (FLCE) are similar to those of medaka HCE (MHCE) and LCE (MLCE) [ 21].
The C346 but not the C370 RNA was highly sensitive to intrinsic cleavage in halted ECs; the positions of cleavage suggested that halted C346 ECs spontaneously backtracked by 4 6 nt.
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