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Furthermore, we investigated the position of alignment of reads within the various rRNA genes, and found that for both libraries, reads aligned along the entire length of these genes (Additional file 7: Table S4).
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This last step generates quality scores ranging from 1 to 10 for each residue in each position of the alignment, and these were used to trim uncertain regions from the alignment.
Based on the position of the alignment, we further filtered for reads that partially aligned within 5 bp of an exon boundary using RefSeq annotation.
Thus when three nucleic acid sequences are aligned there are 4 = 64 possible patterns at each position of the alignment, for example, (AAA, AAG, …).
The error in the position of the alignment markers, measured on the PET images at three different locations in the image, was systematically smaller than 2.3 mm, which corresponds to a displacement of one voxel.
Shannon entropy was used to score the variability at each position of an alignment of HIV-1 circulating sequences [19].
The most 5' position of this alignment was used as a putative transcription start site (TSS).
But in all of them, except Giardia, the R residue is present in the same position of the alignment.
The first, CAT20 [ 26] assigns each position of the alignment to one of 20 pre-computed categories.
The first position of this alignment identifies a base pair where transcription is initiated defining a TSS.
Accordingly, the residues at analogous to H28 position of the alignment are found to be polar/charged in most of the sequences of Clusters II, III, and V.
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CEO of Professional Science Editing for Scientists @ prosciediting.com