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In the present study, the first objective was to identify and position loci associated with sex in S. viminalis by performing quantitative trait locus (QTL) mapping and to determine if the sexual system is male or female heterogametic.
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In particular, each ramet has been genotyped to identify in it n = 8 pairs of alleles, i.e. pairs of microsatellites that occupy a specific position (locus) on the chromosomes, each element of the pair characterizing the same locus in the two homologous chromosomes arising from the maternal and paternal gametes in a sexual reproduction event.
Chromatin remodeling associated to DNA synthesis, CTs relative positioning, loci spatial proximity, intermingling of chromatin loops and transcriptional activity could be critical to determine chromosome damage localisation, genomic instability and cancer-prone translocation frequencies.
Marker selection and map position for loci on chromosomes 1 and X are provided in Table 1 >.
Therefore, these data indicate that both the graphical genotypes and the mapping position of loci were adequate.
Potokina et al. [ 6] combined SNP and other transcript derived markers to position 1596 loci on the Steptoe × Morex [ 7] linkage map.
A total of 241 STRs were positioned at loci that were uniquely identified, and a total of 1748 STRs were positioned at loci that were each redundantly identified (e.g., two STRs with the exact same series of genotypes across the 44 F2 progeny identify the same locus).
The total length of the concatenated dataset including Macrostomum lignano was 6,718 amino acid positions (32 loci), while the concatenated dataset without Macrostomum lignano (43 loci) had 10,218 amino acid positions.
The map positions of loci where both parents were homozygous were added later by imputing them from the relative positions of their homoeologous loci.
2. Position of locus.
Positions of loci PinA and PinB were obtained from published data [ 46].
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