We injected local anaesthetic (lignocaine) into the whisker pads either ipsilateral or contralateral to the hemisphere containing the recording electrodes (which were positioned in layer IV).
With the electrodes positioned in layer IV, higher stimulus intensities elicited larger amplitude LFPs (predominantly the negative component) and larger amplitude decreases (Fig. 8).
With the electrodes positioned in layer IV (based on the maximum evoked LFP amplitude), we established a pre-injection baseline for LFP and responses by giving 10 whisker stimulations (1.2 mA, 0.3 ms).
To investigate responses to sustained stimulation, we applied electrical stimulation to the whisker pad at 10 Hz for 40 s when the electrodes were positioned in layer IV (n = 5 rats).
To investigate the effect of sustained stimulation, we applied a 10 Hz train to the whisker pad for 40 s, with the electrodes positioned in layer IV. (Note that this procedure was performed before the local anaesthetic injection).
Second, we generated input output curves for LFP and responses to a range of stimulus intensities (0 3 mA in 0.5 mA steps) when the electrodes were positioned in layer IV, and then plotted LFP amplitude against the amplitude of the decrease (Fig. 8).
The amplitudes of the [Ca2+]i transients were slightly increased by the application of 2 µM bicuculline, and the decay times were slowed, particularly near the stimulus position and in layer 2/3.
We calculate the apparent position in each layer following a recursive process starting from the second layer until the last layer.
Under the assumptions of the model presented here, in general, the local methanol concentration in the catalyst layer cannot be expressed as an explicit function of the position in the layer.
Concentric bipolar stimulation electrodes were positioned symmetrically in layer II/III of each hemisphere 2 2.5 mm lateral to the midline, and recording electrodes placed 500 µm laterally.
The tracking information of the muons will be extracted from the two-dimensional readout of the RPCs and its position in respective layers along with the upward and downward directionality determined from the timing information.
