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Some governments would prefer to see the position abolished.
Based on a previous study that mapped the residues that contribute to 1D4 binding, it is likely that phosphorylation at the T342 position abolished the 1D4 epitope [20].
Cross-linking the clamp in either the closed or open position abolished transport activity, whereas release of the PPXD with DTT restored it.
Thus, moving the sodium acetate group by just one position abolished the conformational rearrangement of the thiophene backbone observed for HS-68 when bound to these aggregated morphotypes, which confirmed that the anionic substituents must be positioned in a chemically defined fashion to achieve sensitive spectral separation.
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The locked hindfoot position abolishes the normal foot progression through the stance phase of walking and even blocks subtalar inversion and eversion.
Previous site-directed mutagenesis study has identified four conserved residues constituting the active site of Cas1 endonucleases (E141, H208, D218 and D221 in Escherichia coli Cas1); alanine substitutions at any of these positions abolished the nuclease activity of the Cas1 against all substrates tested [ 37].
Removal of the chloro substituents at the carbazole ring 3- and 6-positions abolished activity (ML67-2, Figure 3A, Table 1), whereas the 3,6-dibromo congener ML67-13 showed a slightly increased potency (EC50 177.4 ± 1.1 μM) and a dramatically improved maximum response (efficacy) (Emax ∼20 fold).
He left the paper, and the Post abolished his position.
In both families of enzymes, single point mutations at these positions abolish or greatly reduce microtubule- or actin-stimulated ATPase activity of isolated monomer heads (this report and refs 27, 39, 42-45).
This component is voltage dependent and substitution of the 7′ and 12′ positions abolishes the non-competitive antagonism found in homomeric receptors (Gunthorpe and Lummis, 1999; Thompson et al., 2011a).
Mutations in pUL31 at position Y31 abolished pUL34 binding, whereas mutating position Y34 strongly reduced the interaction as evident in GST pull-down assays.
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