Exact(1)
POS control (Ali et al. 2012) No Yes Digital No N/A Low I 29.
Similar(59)
POS, positive control; WT, wild-type tobacco plant; #n, independent line numbers.
Thus, the fluorescence intensity ratios (Cy5sam*Cy3pos)/(Cy5pos*Cy3sam) will be proportional to the methylation density in the original sequence (sam: sample; pos: positive control).
The 3'UTR region, which is frequently highly conserved among orthologue genes due to its involvement in pos-transcriptional control, displayed an identity above 70% in the human/eutherian mammal comparisons.
For determination of cleaved caspase-3 activity in whole-cell extracts of isolated primary hepatocytes and livers, murine Hepa1-6 hepatoma cells were treated with 50 μm Sorafenib for 24 h and were used as a positive control (pos).
For the detection of cleaved caspase-3 via immunoblot analysis in whole-cell extracts of isolated primary hepatocytes and livers, murine Hepa1-6 hepatoma cells were treated with 50 μm Sorafenib for 24 h and were used as a positive control (pos).. Immunoblots were visualized by Amersham ECL Prime (GE Healthcare) using the Fuji LAS-4000 Mini, according to the manufacturers' protocol.
Lysates of HEK293T cells overexpressing FLAG-PKM1 or FLAG-PKM2 were used as positive controls (pos. ctr).
Lysates of HEK293T cells overexpressing JNK1CA were used as positive controls (pos. ctr) for the detection of phospho-active JNK (p-JNK).
Spots with intensities similar or stronger than positive controls (pos) were selected as ligands of the corresponding SH3-b domain.
KCM contributed greatly to the design of the study, supervised the preparation of the Palmes tubes and performed the tubes pre- and pos-exposure quality control check in the Netherlands.
The repetitive part of the control region (pos. 16,129-16,360 referring to X79547.1 [ 38]) was masked with "N's" as we also discarded this region in the probe design of the array.
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