Exact(13)
There were 45 synonymous DNA substitutions in the sequenced portion of the coding region between the two actin genomic clusters (Figure 4), so that comparisons within a single sequence cluster were distributed around a mean of 9 nucleotides and comparisons between groups were distributed around 54 differences.
In these copies, a portion of the coding region of the parental gene apparently became an intron after retroposition (fig. 2 and supplementary table S1, Supplementary Material online).
MtCBF11 has most likely arisen from an in-frame fusion of the 5′ portion of the coding region from a first CBF/ DREB1 gene with the 3′ portion of the coding sequence from a second gene.
DAM2 primers were (5') TGACTCGGGTGATCGAAAG and (3') AGTCGCTCGTTCTCTTCC and amplified a 313 bp product covering the 3' portion of the coding region (none of which was homologous to DAM1).
In this work we present the results of the analysis of a portion of the coding region of mtDNA, using individuals belonging to extended families from the Azores Islands Portugall).
We amplified and sequenced the genomic sequences of this locus from LA3560 and determined two nucleotide substitutions in its 3′ portion of the coding region by using wo as reference (Accession number: JF518780) (Fig. 1b).
Similar(47)
Furthermore, although the binding was weaker than in the LEE1 operon, Pch bound to other parts of the LEE island: the upstream region of the grlR grlA operon, the 5′ portions of the LEE2 and LEE3 operons, the downstream portion of the LEE3 operon, the upstream portion of tir, the coding region of cesT, and the downstream portion of eae.
We have found that the pattern of mutations that are likely to severely impair gene function because they truncate open reading frames or delete portions of the coding region is not random, suggesting that genetic heterogeneity will be relevant in A. thaliana as it is in humans.
Forward and reverse primers for cloning possessed at their 3′ end 20 25 nucleotides identical to the N terminal and C-terminal portions of the coding region of the targeted ORF, along with Gateway BP reaction compatible recombination sites [ 25].
For all calculations, 2 M1 samples from [ 50] and the 3 M1 from [ 52] were discarded – the first ones were missing several portions of the coding region, and the second ones seemed to exhibit potential sequence errors (See [ 68] for details).
By 24 h of differentiation, the Myog gene is expressed (Rampalli et al, 2007; see Figure 6B) and demonstrates extensive loss of H3K27me3 across the 5′ regulatory region and portions of the coding region (but not at the 3′ UTR), whereas the 5′ end of the coding region becomes enriched for H3K4me3.
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