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Known taste receptors are preferentially expressed in the top portion of taste buds.
TMEM44 transcripts in these cell processes likely account for TMEM44 expression in the top portion of taste buds by microarray analyses (Fig. 2B).
Genes associated with the cell cycle and stem cells are preferentially expressed in the bottom portion of taste buds, suggesting that precursor cells are located there.
Transmembrane protein 44 (TMEM44), a protein with seven predicted transmembrane domains with no homology to GPCRs, is expressed in a TRPM5-negative and PKD2L1-negative population that is enriched in the bottom portion of taste buds and may represent developmentally immature taste cells.
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During histological analyses of a set of TB-associated genes identified from this first microarray set, we discovered that certain transcripts were specifically enriched in cells located at the bottom of the taste bud, suggesting divergent gene expression between the top and bottom portions of taste buds.
The most intense anti-mouse GAT4 immunoreactivity was localized to a few TRCs in the basolateral portion of the taste bud and in the cells surrounding the taste buds.
Moreover, a GABA reuptake system which is critical to the physiological function of a GABAergic signaling pathway is expressed in the basolateral portion of the taste buds, the primary site of interaction between taste receptor cells and their post-synaptic targets.
CALHM3 (Fig. 6A B), CALHM2 (Fig. 6D E), and CALHM1 (Fig. 6G H) were all expressed in FG and CV taste buds with higher levels in the top portion of CV taste buds by microarray analyses.
This overlap in tastes gives you a foothold to share a portion of their taste in music.
SV2B transcripts were expressed in FG and CV taste buds (Fig. 9A) and were enriched in the top portion of CV taste buds (Fig. 9B) by microarray analyses.
Due to these differences in terminology between rats and mice, our GAT4 findings parallel the report of GAT3 in rat taste cells [43], including the localization of the protein at the basolateral portion of the taste buds.
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