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Drive a spade down to detach an offset from the parent plant and lever each one out with a portion of root attached.
E. Portion of root with reddish hairs (bar = 0.2 mm).
TF 6178 preserves a small portion of root very close to the cervix, which dimensions are >3 mm (mesio-distal) by 6.35 mm (labio-lingual).
Here also root was not dissected into three zones but only tip portion of root was taken.
Magnified portion of root systems colonized by P. fluorescens showing P. fluorescences (B ) only or all three reporters together (C ).
Here root was not dissected into three zones but only tip portion of root which showed growth and development was taken.
Similar(54)
In spite of the gorgeous rosette of green leaves adorning each plant are a forgotten, bulky portion of roots.
The latter portion of roots was frozen, lyophilized and ground in a mortar.
The coronal portion of roots was discarded.
Images of whole-root systems (A, D ) or magnified portion of roots (C, F ) at 22 DAS expressing ProACT2 PPYRE8o and ProZAT12 LUC (green, A, B, C ) or ProDR5rev LUC+ (green, D, E, F ). Luminescence from PPyRE8 and LUC reporters visualized together using an open filter setting (visualized in grey-scale) while LUC signal is distinguished using a band-pass filter (517 to 567 nm, visualized as green).
Whether the cellular elements from which the root clusters arise are pre-formed and exist as dormant primordia until evoked by the environment (i.e., they are proleptic primordia), or whether the primordia are developed de novo within barren, pre-existing portions of root axes (i.e., the roots are adventive) is not known.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com