In a portion of nuclei taken from the eighth abdominal segment region of a germ-band elongation stage embryo, FISH signals from the Abd-B m promoter region co-localize with signals from the distal IAB5 enhancer, while the more proximal IAB8 enhancer remains distantly located and disassociated [46].
It is to be noted, however, that in CD-rescued morphants a portion of nuclei maintained an eccentric position, indicative of an imperfect recovery in the formation of myotubes.
Together, these data led us to propose that the early defects in OPMD would depend on PABPN1 function and that aggregate formation might contribute to pathogenesis during later stages when aggregates reach a large portion of nuclei, thus altering nuclear function.
Transmission electron microscopy of apoptotic NIH3T3 cells revealed that portions of nuclei could be observed within membrane blebs.
The histological and morphometric analysis of five brainstem disector pairs containing respiratory neurons located in a region that extends from the compact portion of the nucleus ambiguus to just behind the facial motor nucleus (Fig. 6) ruled out the presence of neuronopathy in DMSXL mice respiratory neurons.
When Lig4-GFP was expressed alone in the wild-type cells using the P41nmt1 promoter, it mainly localized inside the nucleus, with a higher concentration in the nucleolus, the portion of the nucleus not stained by the DNA binding dye Hoechst 33342.
The opposite was observed for connectivity to limbic areas with a maximum reached in the anterior tip of the nucleus, while connectivity to associative areas was highest in the centroinferior portion of the nucleus.
The LPBN is reciprocally connected to different areas, such as area postrema (AP) and medial portion of the nucleus of the solitary tract (mNTS) in the hindbrain and forebrain areas, such as the paraventricular nucleus of the hypothalamus, central nucleus of amygdala and median preoptic nucleus [ 1- 7].
The x-ray zinc fluorescence was also diffuse throughout the nuclei of the majority of cells; but in mitotic cells, it was excluded from the portion of the nucleus with high phosphorus signal (Figure 5B).
Recently, it has been demonstrated that the shell portion of the nucleus accumbens can no longer be considered a homogeneous structure, and can be subdivided into five separate regions.
