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L-amplified product (portion of L) was obtained by using RT-PCR primers L-F4209 (5′-GCGCATTGCAGAGAAAGTC) L-R51135′-CAACGTGATCACCATCTAGAAA-3′AAA-3′) anestedted PCR primers L-F4273 (5′-TGTAAAGTCATGGCCTCAGC-3′) L-R48784878 (5′-CATCCGGGAGAAATTGTCA-3′).
For the portion of L gene sequenced (320 bp), the level of amino acid divergence between the 2 is 17.3%, higher than the level of divergence between other Old World arenavirus species (e.g., 14.5% aa divergence between Mobala and Lassa viruses; Table 2).
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