The portion of cells in different periods can be calculated according to the DNA content.
Figure 2 shows the portion of cells in different periods, which was calculated according to the relationship between the DNA content and the intensity of the PI.
The S228P mutant was prone to form intracellular aggregates for both types of fused proteins, while cells expressing GFP-S228P had a larger portion of cells containing aggregates than cells expressing S228P-GFP (Fig. 1C).
A separate portion of cells was treated with equivalent amounts of isotype control antibodies.
Everyday, a small portion of cells were stained with trypan blue and live cells were counted using the hemocytometer.
The percentage is consistent with the portion of cells entering the cell cycle during a 48-hour activation period.
The results indicated that, compared with the control, N expressing cells had larger portion of cells in sub-G0 phase (23% as against 4%) (Fig. 1d).
The MAb 11A8 bound to a larger portion of cells infected with HIV-1 clade B, with higher mean channel fluorescence, than to uninfected cells (Figure 6).
Importantly, after 24 hours BI 2536 treatment a significant portion of cells became phospho-Histone H3 positive and these were almost all α -actinin negative (Fig. 1C D).
Upon stimulation with platebound anti-CD3+ soluble anti-CD28 mAb, and independent of the concentration used, a large portion of cells was not triggered to proliferate.
Therefore, it seems reasonable to hypothesize that the presence of some portion of cells with "abnormal" PS exposure may provide the effective membrane "landing" of CTs.
