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Because there is no obvious relationship between recall rate and precision rate observed in our analysis and a high portion of annotated inactive hits in the hit list are not our expected result, we regard recall and precision of equal importance in evaluating similarity search performance.
However, the complimentary deoxyribonucleic acid cDNA microarray technique suffers from its inherent high background signals and depends on predesigned probes against known target transcripts, which makes it unable to detect novel transcript regions and it can only cover a portion of annotated transcriptome.
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In this sample 42 (78%) generated a PCR band in an RT-dependent manner, indicating that the co-transcribed TARs represent previously uncharacterized portions of annotated gene models (Figure 3E and 4C).
Third, there is only a small portion of the annotated probe sets for which rat orthologs have been identified.
We are currently pursuing this route and expect that using a larger portion of the annotated corpus for training and testing will improve the classification performance.
However, our transcriptome data have covered a large portion of the annotated transcripts (76.8%, 15 715/20 460) at Ensembl database (http://hgdownload.soe.ucsc.edu/goldenPath/susScr2/chromosomes/), indicating the robust ability of RNA-seq in discovering transcripts with lowly expressed values.
To enable in silico analysis of these probes for possible hybridization with other transcripts, we constructed a Perl script to identify matches of >10 nt in the transcribed portion of any annotated genome (in GenBank or Ensembl format), estimating the Tm of each target and off-target match by the method of Allawi et al.[ 14] as implemented in the BioPerl Primer module.
In others the CNSs map to portions of an annotated D. melanogaster enhancer, with multiple CNSs mapping to the same D. melanogaster enhancer (eAPR, CQ).
In several genome projects, portions of the annotated sequences have been classified in "hypothetical", "conserved hypothetical" or "of unknown function" categories [ 86, 87].
Based on GO-slim functional annotations, this annotated portion of the P. borchgrevinki transcriptome showed widespread differences from the transcriptome of the temperate model fish species D. rerio.
(XLSX 743 kb) Additional file 3: Pathways found in the annotated portion of the transcriptomes.
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