For each time point, bacterial populations were obtained in three separate leaves for each genotype.
Study Design: Decidual macrophage populations were obtained by an enzymic digestion, Ficoll-Paque centrifugation, or flow cytometric sorting.
Synchronized adult nematode populations were obtained as described previously [29].
Four independently mutagenized phage populations were obtained and used for serial passage experiments.
The SNP genotypes for seven populations were obtained from the International HapMap Project [2].
Two different cell populations were obtained by stable lentivirus-mediated transfection of two different SR-BI/CLA-1 shRNA sequences.
For RNAi experiments, populations were obtained from synchronized egg lay on plates seeded with dsRNA-expressing bacteria or control bacteria.
Phylogenetic relationships between island chaffinch populations were obtained by Bayesian inference (BI) using Mr. Bayes v. 3.1.2 [59], [59].
Clonal parasite populations were obtained by limiting dilution series and intravenous injection of one parasite in 10 recipient NMRI mice.
Cloned lines of the resistant parasite populations were obtained for both experiments (i.e. clone Δp52-1 and Δp52-2) by the method of limiting dilution.
G2/M populations were obtained following 16 hours (hrs) of treatment with nocodazole (200 ng/ml) used to prevent cells from cycling.
