JC-1 monomer-positive cell populations were determined with a FACSCalibur instrument.
Confidence intervals based on the means of wild-type, heterozygote, and mutant sample populations were determined.
Fractal dimensions for characteristic aggregate populations were determined to be around 1.8, and were independent of combustor axial location.
In one square, changes in protozoal populations were determined using microscopic counts and denaturing gradient gel electrophoresis (DGGE), whereas changes in bacterial populations were determined using DGGE and ribosomal intergenic spacer length polymorphism (RIS-LP).
Microbial counts for total bacteria, enterobacteria and lactobacilli populations were determined by quantitative PCR using SYBR Green® dye.
Stationary-phase cells were inoculated into sterile media to give initial populations of 6 7 log10 CFU/ml and bacterial populations were determined periodically by aerobic plate counts.
For stable 0.5, 2.5 and 5.0 mg/mL TiO2 dispersions in the presence of 250 × 10− 6 mol/L Gemini surfactant (ζ-potential ~ 40 mV) only two scattering populations were determined, at 78 95 nm and at ~ 280 nm.
The genotypes of F2 populations were determined by using SNP markers selected from genome-wide SNP marker data (Yonemaru et al. 2014a) and SSR markers selected on the basis of the data from the International Rice Genome Sequencing Project (2005).
For gene expression studies, since a normal distribution of the values determined was not found, data are depicted as medians with interquartile range and statistical differences among populations were determined using Kruskal Wallis with Dunn's multiple comparison test.
At lower temperature of about 100 K only six conformers were significantly populated according to their electronic energies while at higher temperature of about 400 K the populations were determined by their vibrational partition functions.
The lysine contents in kernels of parents, F1, and F2 populations were determined using near infrared reflectance spectroscopy (NIRS) method, and analyzed in the model of triploid seed endosperm-cytoplasm-maternal effect.
