Populations of strains are identified by different colours: WildAsiaSiev (red), AgroAsiaSiev (orange), AgroAsiaDom (purple), AgroEuDom (yellow) and WildEuSylv (green).
Based on multilocus sequence analysis of a panel of 370 H. pylori strains isolated from humans in different parts of the world, seven populations of strains with distinct geographic distributions have been identified [ 17].
Strains repeatedly unable to carry out self-fertilization were defined as gonochoristic – in all cases, mass populations of strains found to be gonochoristic by this criterion were also observed to contain large numbers of mating males.
However, it is worth mentioning that after 110 days a remaining bacterial population of strain G18T was still observed.
The PCR products amplified from the genome of strain M4-2A contained multiple fragments, suggesting that a population of strain M4-2A harbored partial deletions in the integrated expression cassette of the PeRBCS promoter– PeUMPS cDNA the PeRBCS terminator.
Growth rates of isolated clones from the end-point populations of (a) strain E and (b) strain F. Growth rates of each 10 isolated clones and population under 5%% ethanol stress conditions are presented with that of the parent strain.
Moreover, LacS-PCR-SSCP analysis of the 29 strains provided 2 different profiles, whereas 4 strain-specific profiles were detected by LacS-PCR-DGGE, indicating the potential to use these techniques for profiling and monitoring population of strains of S. thermophilus in food products.
Indeed, the population of strains with the significantly highest ability to bind to fibrinogen had both the fbsB and fbsA genes and belonged to CC17 phylogenetic lineage [17].
MLST results also suggested that the population of strains reacting with all three of the 9, 31, and 32 antibodies is heterogeneous and it is likely that these strains represent a diverse population carried in healthy pigs (data not shown).
This indicates that the consensus genome of the population of strains is gradually approached.
