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The largest population is selected for lowess normalization [ 29] generating a population specific correction curve (step 6, Figure 5).
The extrapolation is done conservatively in the end points of A by using the first/last data point of the population specific correction curve to level out the global correction curve horizontally in the M-A plot thereby moderating the impact of extreme points or missing values.
The pharmacodynamic relationship between treatment with moxidectin and the Q wave-T wave (QT) interval in an ECG was investigated graphically using plots that showed the time-course of moxidectin and QT correction (QTc), based on Fridericia's formula (QTcF) and a population-specific QT correction (QTcN) formula.
Second, when considering only the CNVs with population-specific frequency changes, 10 out of 14 (71%) transcripts showed significant expression differences among populations (Kruskal-Wallis Test; P<0.0036; after Bonferroni correction; Table 3) and we could assess a positive correlation between expression levels and the copy number in 60% of them (6 out of 10) (Figure S2).
Correction for population substructure in large scale studies minimises the rate of false positives and dilution of a population-specific signal.
But some population-specific variants may exist, and a population could fail to benefit if the variant is not detected.
Population-specific approaches abound as well, such as Omada for diabetes or ConsejoSano for Hispanic populations.
Accordingly, we cannot exclude population-specific effects.
Population-specific transcript networks.
This uniform background may also affect the random correction process, but no specific correction was applied to account for.
The raw data files underwent background correction, normalization, probe specific correction, and summary value computation using the Robust Multichip Average (RMA) method in the Bioconductor package.
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