The correlation among the grain shape and chalkiness parameters in the RIL population was analyzed (Table 1).
The single-cell population was selected on a forward-side scattergram, and the green fluorescence from this population was analyzed.
The physiological and the energetic state of the averaged cell population was analyzed by biomass/substrate yield (Yx/s), biomass specific substrate uptake rates (qs), and adenylate energy charge measurements (AEC), each measured at steady state growth conditions (Figure 2).
The RI population was analyzed with leaf gas exchange techniques for photosynthetic CO2 assimilation rate (A/m2), the ratio of intercellular to ambient CO2 concentration (Ci/Ca), and SCO.
Fecal Microbial population was analyzed using group-specific primers (Bartosch et al. 2004; Franks et al. 1998; Koike and Kobayashi 2001; Lee et al. 1996; Matsuki et al. 2002, 2004; Rinttila et al. 2004), as listed in Additional file 1: Table S1.
DNA extracted from plucked eyebrow hairs collected from the study population was analyzed with a cutaneous HPV subgroup polymerase chain reaction and newly designed HPV type specific polymerase chain reactions for HPV 2, 5, 8, 15, 16, 20, 24, and 38.
Percentage of the ALDH positive population was analyzed by ALDEFLUOR assay.
CD4+ T cells population was analyzed using CellQuest software (BD Biosciences, CA).
Purity of the resulting cell population was analyzed by FACS to be >98% (data not shown).
To verify depletion, the remaining cell population was analyzed by flow cytometry which consistently revealed purification grades of >95%.
As previously described [14], the cell characteristic forward-scatter pattern was used to generate a gate and only this population was analyzed.
