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These cells fulfill the in vitro and in vivo criteria for stem cell activity including the ability to reconstitute a heterogeneous population, sphere- and colony-forming activity, and the ability to form tumors in immune deficient mice.
Even in the N1-ICD-expressing CD44+CD24low+ population, sphere formation and tumour growth were not fully abrogated by GSI treatment.
9 These characteristics can be identified using standardized in vitro functional assays: detection of the side population, sphere formation assays, and clonogenicity assays, for example.
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Moreover, we observed that, compared with the total DPC population, sphere-forming DPCs expressed higher levels of Nestin and P75 and secreted greater amounts of GDNF.
This calculation, as mentioned above, gives the precise number for a uniform population of spheres with equal diameter.
Moreover, the enrichment of this sub-population during sphere formation was almost completely prevented by ATG4 A inhibition.
To determine the CFE of each cell population, tumor spheres of at least 60 μm were counted on day 9 after plating.
Bursts are of a fairly uniform and regular intensity – a desirable feature as it is from a uniform population of spheres.
EM image analysis yields cross sections of such a heterogeneous population of sphere-like vesicles at random levels, occasionally including sections through their largest diameter at their centers.
In the present study, we focused on miRNAs of GEM-resistant pancreatic cancer cell lines and CSC-like cell populations using sphere formation assays (Pastrana et al, 2011); pancreatic CSCs could be enriched as spheroid cells (Hermann et al, 2007).
The total volume of the population of spheres in a cell with 5000 μm size (the approximate average size of a rat hepatocyte [ 4]) will be 5000 × 0,0041 = 20,5 μm.
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