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As a comparison with our new SNP typing approach, we compared parentage assignment for eight insurance population offspring that were also genotyped using microsatellites.
In this regard, the high-throughput SNPs are more powerful in evaluating the authenticity of the DH population offspring than other regular markers.
Most importantly, the two traits known to be heritable in the Cueva del Azufre population (offspring size and fecundity [ 53, 55]) did not show a flood signal at all, suggesting a lack of immigrants from the other habitat types.
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Nevertheless, we also found differences in the performance of the within-population offspring among the four experimental populations.
One example of this is where females of the red-colored Moliro population have been shown to discriminate strictly against the blue-colored males from the Chaitika and Nakaku populations (Egger et al., 2008; Fig. 1), although cross-population offspring produced in no-choice situations is viable and fertile (Sefc, pers. observation).
As it can be observed in Figure 2, experimental conditions, which were intended to be close to real TOS habitat conditions, were especially favourable for TUR, since within-population offspring for this pond presented very high values for this fitness component.
The familial populations, offspring with affected siblings or with affected parents, accumulated 865,475 and 4,751,609 person-years at risk, respectively; the average follow-up time was 14.8 years.
In the N2 populations, offspring fecundity decreased with increasing level of inbreeding; inbreeding level had no effect on offspring fecundity in the N10 and N40 populations (Fig. 1B; Table S5).
In the N2 populations, offspring fecundity was lower than in the control population at three of six inbreeding coefficients (at f = 0.26, f = 0.38 and f = 0.68; mean value <1, CI not overlapping 1; Fig. 1B; Table S3).
Offspring aged 30 59 years and living locally (in 1996) formed the eligible population (3202 offspring from 1767 families).
The Landrace population comprised offspring of 56 boars crossed with 264 sows where the largest half sib family included 24 offspring.
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