It is plausible that a decrease of neural activity in specific segments or neuronal populations of the brain could lead to the loss of information flow and there is experimental evidence to support this hypothesis [20].
Our technique guarantees the isolation of sufficient high quality RNA obtained from specific cell populations of the brain expressing soluble fluorescent marker, which is a critical prerequisite for subsequent gene expression studies by microarray analysis or qRT-PCR.
This finding is not too surprising as it is thought that the products of these genes are primarily responsible for bringing protein clients to chaperone activities [93]; and one might expect significant diversity in regulating the folding of both unique and ubiquitous protein clients across the many diverse cell populations of the brain.
To identify DAergic neurons for study, we used ETvmat2 GFP zebrafish that express GFP in monoaminergic cell populations of the brain.
Further experiments involving isolation of individual cell populations of the brain or retina will be needed to determine the specificity of these DHSs.
Whereas diversity among neuronal populations of the brain is well-understood (Klausberger and Somogyi 2008), little is known about the origins of astrocyte subtypes.
Here, genes encoding neurotrophins might be considered as the most attractive candidates, because these proteins and their receptors are expressed in the neuronal populations of the brain undergoing synaptic plasticity and also participate in neuronal development, synaptogenesis, and response to stress/anxious stimuli [18].
DOI: http://dx.doi.org/10.7554/eLife.02439.018 Astrocytes throughout the brain arise from radial glia, the primary multipotent neural precursor cells population of the developing brain (Kriegstein and Alvarez-Buylla, 2009).
The dynamics of the population activities of the brain regions, the DA release in the striatum, and D2 receptor occupancy are given by (5) The symbols used in the equations are also listed in Table 1.
As wild-type flies aged (60 days after eclosion, corresponding to ∼80% mortality of the population), examination of the brains of surviving flies revealed a low frequency of vacuolar lesions; when present, these lesions were distributed homogeneously throughout the brain (Fig. 1B,D).
