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Microsatellite-based DNA parentage analyses were used to assign oysters to families and population of origin.
Complete modern mtDNA genomes from Finland were obtained through GenBank searches, HmtDB searches15, 16 and from published literature, including information on the population of origin.
Genetic assignment of fish captured in inlets to their population of origin was performed, and marine migration patterns were inferred from the genetic assignments.
Damage significantly affected plant architecture (an increase in number of stems/plant height), and there was no differential effect of damage according to population of origin.
To assess the impact of sQTLs on transcription rate, we tested, for each sQTL, the effect of the peak sQTL SNP on intronic RPKM in the condition where the sQTL was the most significant, adjusting for population of origin.
If the population of origin of an individual is not represented in the sampled set of reference populations, the assignment algorithms will still designate a population of origin, albeit an incorrect one [6].
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Testing the population divergence vector revealed that population-of-origin had the strongest effect (= 0.37) followed by parents (= 0.20).
Population-of-origin had strong effects on overall shape variation as well as the population and predator divergence vectors.
No significant interaction was found between sex and population-of-origin (Wilks' Lambda = 0.832; F18,331 = 1.236; p = 0.230).
Collectively, these results indicate that body shape variation among offspring was most strongly influenced by their population-of-origin, followed by predator-induced phenotypic plasticity.
This study illustrates the great potential of patterns obtained from ancient DNA to identify population-of-origin to investigate historic genotype structure of extinct populations.
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