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Not isolated from each other, these invisible networks are intertwined and linked through complex flows of population, materials and information.
SNP discovery rate: one or more SNP discovered/PCR fragments analysed in the small population materials.
Despite their apparent usefulness as genetic markers, only a moderate number of Salmo salar SNP markers have been identified and validated by genotyping in population materials [ 5- 11].
The F2 population materials including two F0 parents and 277 F2 progeny were gained from the rice genetic breeding laboratory of China Agricultural University.
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The book's central point, much criticised since, is that "the earth is finite" and the quest for unlimited growth in population, material goods etc would eventually lead to a crash.
The current paper is based on total population material from both countries.
In this step individuals from the small population material were genotyped by sequencing the amplified target gene fragments.
The population material screened when searching for SNPs in the 165 PCR fragments consisted of relatively few individuals.
In Ryynänen and Primmer a population material consisting of a similar small number of individuals were screened when searching for SNPs [ 6].
Thus, only 61 PCR products (47%) from 57 different target genes could be further used for SNP search in the population material.
The final SNP discovery step was, like in the UTR-primed method, performed by genotyping individuals in a small population material.
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