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The complete set of (supported or unsupported) non-inferior solutions, consisting of combinations of multi-compartment containers for the disposal of four types of sorted waste in nineteen candidate sites, and population assignments, was generated.
Approaches that compare allele frequencies across populations in order to determine population assignments have been successfully used with MLST data from B. pseudomallei and other highly recombinogenic species.
Separate analysis of the two North American populations nonetheless reveals k = 2 as the most likely number of clusters, and population assignments coincide perfectly with geographic locations (Figure 1).
Briefly, 100,000 iterations with a burn-in period of 30,000 iterations were used to determine population assignments of STs using the "admixture" model and assuming two populations as previously established [7].
To evaluate the validity of population assignments and to identify the possible range of q-values for potential purebreds and different hybrid classes, a series of simulations were conducted for parental, hybrid, and backcrossed genotypes [13], [35].
When additional reference population data become available either through expanded sampling across space (e.g. broader geographic coverage of contemporary distribution) or time (e.g. recently extinct species or extirpated populations), reanalysis of population assignments for individuals of unknown ancestry may be warranted.
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The population assignment test correctly assigned 80.59% of the individuals to their original population.
Because genetic divergence decreases due to gene flow after secondary contact, we restricted this analysis to our genetically pure parental populations, by combining all the "pure" individuals assigned to the same population (assignment pp≥0.95) and calculating Nei's D [ 59] with GENETIX [ 60].
The model with three populations performed poorly in assigning samples of the Water Rail to two clusters, the highest population assignment probability for membership of an individual to clusters 2 and 3 being only 0.571 (0, 1.0).
We used the population assignment tests implemented in BAPS and STRUCTURE and two algorithmic species delimitation procedures (ABGD, GMYC) to generate species boundary discovery hypotheses, which were subsequently tested using Bayes factors.
However, the percentage of isolates with trichothecene genotypes that were not predictive of their genetic population assignment (recombinant genotypes) increased from 10% in 2005 to 17% in 2007, indicating that trichothecene type became an increasingly unreliable marker of population identity over time.
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