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As shown in Figure 8, fibroblast adhesion was minimal in uncross-linked multilayers and any observed cells were poorly spread.
In some instances, antibodies to lamin-B1 (Abcam) were used to visualize the nuclear membrane to facilitate the identification of binucleation in poorly spread cells.
By contrast, the NESH/Abi-3-expressing cells did not exhibit lamellipodial protrusions and were poorly spread on the dish at 30 min.
We verified that conditions permitting condensation similarly resulted in poorly spread complexes that could not be used to measure contour lengths and resulted in poor imaging conditions owing to salt and aggregates on the mica.
Cells that were more extensively spread on higher densities of Tol-OP-ST exhibited significantly (P < 0.01) higher levels of BMP-induced signalling compared to cells that were poorly spread and showing fewer adhesion complexes on low density Tol-OP-ST.
On 44 hours (after addition of serum)), the cell started to proliferate and spread; cells cultured on P-COL substrate adopted elongated morphology, while those cultured on T-COL exhibited a more polygonal shape; in comparison, much less cells adhered to TCPS, and cells cultured on TCPS were poorly spread, implying immature cellular cytoskeleton assembly.
Similar(54)
In contrast, on elastin, endothelial cells attached poorly, did not spread, and had markedly impaired functional properties.
Studies conducted by Dr. Heymann and colleagues in Africa showed that monkeypox was not a significant threat because it spread poorly among humans.
Efforts to promote a visit by local government officials to an elderly pensioner in the eastern province of Anhui took an awkward turn after a poorly doctored photo spread rapidly on Chinese websites.
Listings are poorly described and spread across many different sites.
Shattil and co-workers have reported PKCθ-deficient platelets spread poorly on fibrinogen, suggesting that PKCθ positively regulates outside-in signalling.
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