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Those who had two copies of the poorly binding version thought they tasted fine.
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Also, standard coatings are not suitable because the cells bind poorly or binding is toxic (such as poly-lysine substrates).
Rnd3 can down-regulate RhoA.GTP levels by activating p190RhoGAP via an as yet poorly defined binding domain [3]; a complementary mechanism would be to inhibit RhoGEFs such as Syx (Figure 6).
Genes with either conserved or poorly conserved binding sites for a miRNA were defined as targeting genes of interest for the corresponding miRNA.
Within the replication fork, the β sliding clamp interacts with a large number of enzymes, which share a short and poorly conserved binding motif (consensus: Q-L-S-L-F/L, Q1, and L4 being the most strongly conserved residues) (Dalrymple et al. 2001).
To validate this network we have cloned six poorly characterized Ras binding domains (RBD) and two Ras proteins (RERG, DiRas1).
The difference between these two Inr elements was confirmed by our finding that the VP16 initiator element binds to the 65-kDa YY1 transcription factor, and the VP5 Inr element competes poorly for the binding between the VP16 element and infected cell proteins in comparative bandshift assays.
A poorly conserved Rep binding element allows Rep5 to interact with the elongated ITR5 spacer (Fig. 4B).
These loci may amplify erratically due to their poorly matching primer binding sites, thereby reducing the overall reliability in this set.
Initial experiments under our standard conditions (final ratio of ligand to protein 2 1) were inconclusive, thus suggesting a possible poorly defined weak binding (not shown).
In vitro, ATR competed poorly against estradiol binding to cytosolic receptors, with an approximate IC50 of 10 -5) M. Atrazine administration to SD and Fischer-344 (F-344) rats for 12 months, up to 400 ppm in food, was correlated with significant alterations of estrous cycling activity; but there was a divergent strain response.
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