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However, progress in cell therapy is hampered by poor viability of implanted cells and the vulnerability of regenerated tissue to repeated bouts of ischemia.
Accordingly, we never observed complete suppression of either eIF5A or Amd1 in lymphomas driven by their corresponding shRNAs (Supplementary Fig. 5a, b), and primary B cells with near-complete suppression had poor viability (Supplementary Fig. 17).
Embryonic stem cells (ESCs) have been used as an alternative cell source for cardiac repair; however, dissociated ESCs show poor viability in the scaffold and do not form the embryoid body (EB -like structurEB -like
However, the outcomes of existing treatments have not been satisfactory owing to suboptimal localization to implantation site, poor viability, low engraftment efficacy and lack of functional remodeling of the delivered cells.
However, the cells cultured on bare TNAs exhibited a poor viability (48%%).
Previous attempts to culture live prostate tissues resulted in poor viability and lost "tissue architecture," the researchers said, making them less than useful for research or therapy development.
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Unfortunately, the founder mice derived from inbred ES cells, such as the B6 strain, show poor viabilities or abnormalities due to developmental defects (Eggan et al., 2001; Nagy et al., 1993).
Although the second-gen NanoVelcro-LCM approach possesses a great precision in single-CTC isolation, it suffers from a labor-consuming process and poor viabilities of the isolated CTCs.
In contrast, NFAT5−/− cells failed to induce osmoprotective genes and exhibited poorer viability.
Altogether, these results indicated that hypertonicity triggered a rapid cell cycle arrest in both wild-type and NFAT5−/− T cells, but while the former induced osmoprotective gene products and resumed proliferation with only a moderate viability loss, NFAT5−/− cells had poorer viability, and those surviving exhibited cell cycle defects.
In parallel experiments, we observed that NFAT5−/− T cells exposed to hypertonicity during 24 hours exhibited poorer viability (56%) than wild-type cells (70%) (Fig. 1B), and those surviving displayed cell cycle defects, with cultures having a lower proportion of cells in S, and a greater accumulation in G1 and G2/M (Fig. 1C).
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