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For quality category, the four samples were divided into two groups- good varieties (i.e. good chapatti) and poor varieties (poor chapatti).
Normalization and quantification of relative changes in gene expression or fold changes between one good and one poor varieties were calculated by using the following formula: FC = 2-Δ (ΔCt)[ 46].
In cluster III, there was a single probe set (Ta.2025.1.S1_at, oxi-reductase, a Fe (II) oxygenase family protein) showing up-regulation in poor varieties at 14 and 28 DAA.
Cluster analysis of the 110 probe sets into 5 groups identified the co-expressed genes having similar expression patterns in good and poor varieties in three seed developmental stages (see Additional file 5, Figure 3).
The activities of other metabolic pathway genes were more differentiated at 7 DAA between good and poor varieties, but less at later stages (14 and 28 DAA) of seed development.
The spatial distribution of the differentially expressed genes (at least 10-fold between good and poor varieties) was studied in ten growth stages, namely, germination, seedling growth, tillering, stem elongation, booting, inflorescence emergence, anthesis, milk development, dough development, and ripening.
Similar(47)
An insect protection gene will not make a poor variety a high yielder any more than spiffy shoes will turn a slow runner into Usain Bolt.
These people do not keep a variety livestock, so they are not eating enough protein rich meat or fish, and a poor variety of fruit trees means that they are not getting enough Vitamin C".
The survey disproves the notion that the waters in chilly polar regions have a much poorer variety of fauna.
Choose crops you love eating and if you are time poor, choose varieties that require little work.
Using gene-specific two-way ANOVA analysis, 110 probe sets were identified for processing quality showing at least 10-fold differential expression between good and poor quality varieties.
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